Agilent 6400 Series Triple Quadropole Mass Spectrometer

6400 Series QQQ Vent and Clean Procedure (In Progress)

The instructions provided should be used to supplement the official 6000 Series LC/MS System Maintenance Guide. Go to Agilent.com, click on Resources on the menu near the top, select library and then user manuals. Search for 6400 Series LC/MS System Maintenance Guide.

What you will need:

Sonicator Bath

Di H2O

LCMS grade H20

LCMS grade Methanol

Clean glass beakers, various sizes from 200 to 1000 mL

Clean glass graduated cylinder, 100 mL

Liquidnox (www.alconox.com)

T8, T20, Torx screwdrivers

1.5mm ball screwdriver

Canted Spring Coils (2)

Ion Transfer Capillary

* These do not need to be replaced every vent and clean. Consult your service engineer or Agilent for the change intervals. The guide will include instructions on how to change the coils in the desolvation assembly and if replacing out the ion transfer capillary with a new one, you obviously do not need to clean it.

Ample space in front of the mass spec, behind the mass spec and ample table space to set parts on.

Ample time to perform the procedure (2-4 hours to perform, overnight to pump down).

Vent the system

Make sure you are not running any samples and there is no flow into the mass spectrometer. Using the Mass Hunter Data acquisition program, vent the system by right clicking within the QQQ Section and selecting vent.

When selected, a dialog box appears with the following message: If you are sure you want to proceed, click yes. When the process is complete another dialog box appears: It is now safe completely power off the instrument. Start by switching off the power button front cover of the mass spec. Next, unplug the main power cable. The location is behind the mass spec and the main power cable is the lower left one. The cable is removed here.

Locate your vacuum foreline pump, switch off the power to the vacuum and then unplug it from the back of the Mass Spectrometer. Next, grasp the handle on the front cover and pull it towards you.